Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential.
Identifieur interne : 000033 ( Main/Exploration ); précédent : 000032; suivant : 000034Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential.
Auteurs : B Ben Abdelmoumen Mardassi [Tunisie] ; A. Béjaoui Khiari ; L. Oussaief ; A. Landoulsi ; C. Brik ; B. Mlik ; F. AmounaSource :
- Veterinary microbiology [ 0378-1135 ] ; 2007.
Descripteurs français
- KwdFr :
- ADN bactérien (composition chimique), Animaux (MeSH), Anticorps antibactériens (métabolisme), Antigènes bactériens (biosynthèse), Antigènes bactériens (génétique), Antigènes bactériens (immunologie), Clonage moléculaire (MeSH), Dindons (MeSH), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Immunotransfert (méthodes), Infections à Mycoplasma (diagnostic), Infections à Mycoplasma (médecine vétérinaire), Maladies de la volaille (diagnostic), Maladies de la volaille (microbiologie), Mycoplasma meleagridis (génétique), Mycoplasma meleagridis (immunologie), Spécificité d'espèce (MeSH), Séquence nucléotidique (MeSH), Sérums immuns (métabolisme).
- MESH :
- biosynthèse : Antigènes bactériens.
- composition chimique : ADN bactérien.
- diagnostic : Infections à Mycoplasma, Maladies de la volaille.
- génétique : Antigènes bactériens, Escherichia coli, Mycoplasma meleagridis.
- immunologie : Antigènes bactériens, Mycoplasma meleagridis.
- microbiologie : Maladies de la volaille.
- médecine vétérinaire : Infections à Mycoplasma.
- métabolisme : Anticorps antibactériens, Sérums immuns.
- méthodes : Immunotransfert.
- Animaux, Clonage moléculaire, Dindons, Données de séquences moléculaires, Spécificité d'espèce, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals (MeSH), Antibodies, Bacterial (metabolism), Antigens, Bacterial (biosynthesis), Antigens, Bacterial (genetics), Antigens, Bacterial (immunology), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Bacterial (chemistry), Escherichia coli (genetics), Immune Sera (metabolism), Immunoblotting (methods), Molecular Sequence Data (MeSH), Mycoplasma Infections (diagnosis), Mycoplasma Infections (veterinary), Mycoplasma meleagridis (genetics), Mycoplasma meleagridis (immunology), Poultry Diseases (diagnosis), Poultry Diseases (microbiology), Species Specificity (MeSH), Turkeys (MeSH).
- MESH :
- chemical , biosynthesis : Antigens, Bacterial.
- chemical , chemistry : DNA, Bacterial.
- chemical , genetics : Antigens, Bacterial.
- chemical , immunology : Antigens, Bacterial.
- chemical , metabolism : Antibodies, Bacterial, Immune Sera.
- diagnosis : Mycoplasma Infections, Poultry Diseases.
- genetics : Escherichia coli, Mycoplasma meleagridis.
- immunology : Mycoplasma meleagridis.
- methods : Immunoblotting.
- microbiology : Poultry Diseases.
- veterinary : Mycoplasma Infections.
- Animals, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Species Specificity, Turkeys.
Abstract
A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM.
DOI: 10.1016/j.vetmic.2006.08.008
PubMed: 16973309
Affiliations:
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Le document en format XML
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Boutheina.mardassi@pasteur.rns.tn</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire des Mycoplasmes, Institut Pasteur de Tunis, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunis</wicri:regionArea><placeName><settlement type="city">Tunis</settlement><region nuts="2">Gouvernorat de Tunis</region></placeName></affiliation></author><author><name sortKey="Bejaoui Khiari, A" sort="Bejaoui Khiari, A" uniqKey="Bejaoui Khiari A" first="A" last="Béjaoui Khiari">A. Béjaoui Khiari</name></author><author><name sortKey="Oussaief, L" sort="Oussaief, L" uniqKey="Oussaief L" first="L" last="Oussaief">L. Oussaief</name></author><author><name sortKey="Landoulsi, A" sort="Landoulsi, A" uniqKey="Landoulsi A" first="A" last="Landoulsi">A. Landoulsi</name></author><author><name sortKey="Brik, C" sort="Brik, C" uniqKey="Brik C" first="C" last="Brik">C. Brik</name></author><author><name sortKey="Mlik, B" sort="Mlik, B" uniqKey="Mlik B" first="B" last="Mlik">B. Mlik</name></author><author><name sortKey="Amouna, F" sort="Amouna, F" uniqKey="Amouna F" first="F" last="Amouna">F. Amouna</name></author></analytic><series><title level="j">Veterinary microbiology</title><idno type="ISSN">0378-1135</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antibodies, Bacterial (metabolism)</term><term>Antigens, Bacterial (biosynthesis)</term><term>Antigens, Bacterial (genetics)</term><term>Antigens, Bacterial (immunology)</term><term>Base Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>Escherichia coli (genetics)</term><term>Immune Sera (metabolism)</term><term>Immunoblotting (methods)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycoplasma Infections (diagnosis)</term><term>Mycoplasma Infections (veterinary)</term><term>Mycoplasma meleagridis (genetics)</term><term>Mycoplasma meleagridis (immunology)</term><term>Poultry Diseases (diagnosis)</term><term>Poultry Diseases (microbiology)</term><term>Species Specificity (MeSH)</term><term>Turkeys (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (composition chimique)</term><term>Animaux (MeSH)</term><term>Anticorps antibactériens (métabolisme)</term><term>Antigènes bactériens (biosynthèse)</term><term>Antigènes bactériens (génétique)</term><term>Antigènes bactériens (immunologie)</term><term>Clonage moléculaire (MeSH)</term><term>Dindons (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (génétique)</term><term>Immunotransfert (méthodes)</term><term>Infections à Mycoplasma (diagnostic)</term><term>Infections à Mycoplasma (médecine vétérinaire)</term><term>Maladies de la volaille (diagnostic)</term><term>Maladies de la volaille (microbiologie)</term><term>Mycoplasma meleagridis (génétique)</term><term>Mycoplasma meleagridis (immunologie)</term><term>Spécificité d'espèce (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Sérums immuns (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antigens, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Bacterial</term><term>Immune Sera</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes bactériens</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="diagnosis" 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nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country><region><li>Gouvernorat de Tunis</li></region><settlement><li>Tunis</li></settlement></list><tree><noCountry><name sortKey="Amouna, F" sort="Amouna, F" uniqKey="Amouna F" first="F" last="Amouna">F. Amouna</name><name sortKey="Bejaoui Khiari, A" sort="Bejaoui Khiari, A" uniqKey="Bejaoui Khiari A" first="A" last="Béjaoui Khiari">A. Béjaoui Khiari</name><name sortKey="Brik, C" sort="Brik, C" uniqKey="Brik C" first="C" last="Brik">C. Brik</name><name sortKey="Landoulsi, A" sort="Landoulsi, A" uniqKey="Landoulsi A" first="A" last="Landoulsi">A. Landoulsi</name><name sortKey="Mlik, B" sort="Mlik, B" uniqKey="Mlik B" first="B" last="Mlik">B. Mlik</name><name sortKey="Oussaief, L" sort="Oussaief, L" uniqKey="Oussaief L" first="L" last="Oussaief">L. Oussaief</name></noCountry><country name="Tunisie"><region name="Gouvernorat de Tunis"><name sortKey="Mardassi, B Ben Abdelmoumen" sort="Mardassi, B Ben Abdelmoumen" uniqKey="Mardassi B" first="B Ben Abdelmoumen" last="Mardassi">B Ben Abdelmoumen Mardassi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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